Regulation of energetic metabolism by adenosine during parasitic wasp infection. Tomas Dolezal^1,2, Adam Bajgar², Katerina Kucerova². 1) Microbiology and Immunology, Stanford University School of Medicine, Stanford, CA; 2) Department of Molecular Biology, Faculty of Science, University of South Bohemia in Ceske Budejovice, Czech Republic.

   Immune response is energetically demanding process and precise regulation is crucial since both over- and under-stimulation of energy release may have deadly consequences. Although there is a lot of information about the changes of metabolism itself during immune response, we know very little about how these changes are regulated. We are using parasitic wasp infection of Drosophila larvae as a model to study effects of extracellular adenosine during infection. Our results show that the adenosine signaling is required for a circulating glucose increase during infection and that a blocking of this effect results in a decrease of immune cells proliferation and differentiation which in turn affects the outcomes of immune response against the parasite. We are further using this model to dissect mechanisms of the adenosine production and signaling during immune response. Our model has a potential to demonstrate for the first time that the extracellular adenosine is perfectly suitable signal for very dynamic and fine-tuned regulation of energetic metabolism during immune response.