Vienna Tiles (VT) GAL4 driver lines: New resources at the Vienna Drosophila RNAi Center (VDRC). Lisa A. Meadows^1*, Dickson Group², Stark Group², VDRC Team¹, Alexander Stark², Barry Dickson². 1) Vienna Drosophila RNAi Center, Campus Science Support Facilities, Vienna, Austria; 2) IMP - Research Institute of Molecular Pathology, Vienna, Austria.

   The Vienna Drosophila RNAi Center (VDRC) maintains and distributes resources for the Drosophila research community. In addition to our transgenic RNAi stocks, covering more than 93.8% of Drosophila genes, we have recently expanded our resources to include a new collection of GAL4 driver lines, known as the Vienna Tiles (VT) library. This complementary resource has been generated by the groups of Barry Dickson and Alex Stark (IMP, Vienna). It currently consists of >6,000 transgenic Drosophila lines containing short fragments of genomic DNA controlling GAL4 expression. The constructs are integrated at a single defined genomic position (attP2) and each carries a distinct candidate cis-regulatory DNA fragment, typically around 2kb in size.
   These lines have been constructed according to the methods developed by the Rubin group at Janelia Farm Research Campus and form a complementary resource to the Janelia FlyLight collection. The precise expression patterns of reporters driven by the VT lines have been extensively documented and annotated in the Drosophila adult brain (Dickson group, BrainBase) and all lines have been spatio-temporally characterised in embryos (Stark group, www.starklab.org). A database of the expression patterns and their annotations will be available, linked directly to the VDRC website (www.vdrc.at).